2018-03-08 16:05:152025-05-22 12:24:45

locus

BSU18250

BSU_18250

geneLength

1647

1650

outlinks

bsu

BSU18250

BSU_18250

Gene

Coordinates

1,954,525 → 1,956,174

1,954,525 1,956,174

Biological materials

Mutant

MGNA-B087 (yngI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1086 NBRP B. subtilis, Japan]

available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]

BKE18250 (Δ[[gene|yngI]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA

BKK18250 (Δ[[gene|yngI]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA

MGNA-B087 (yngI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1086 NBRP B. subtilis, Japan]

available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]

BKE18250 ([[gene|yngI]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA

BKK18250 ([[gene|yngI]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC, downstream forward: _UP4_TCATCAAGTCAATGAGGTGA

References

15699190, 12662922, 19935659, 20797616

15699190, 12662922, 19935659, 20797616, 22560731

The protein

Protein family

[SW|ATP-dependent AMP-binding enzyme family] (according to UniProt)

The protein

Structure

[PDB|3R44] (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 32% identity) [pubmed|22560731]